prrl ws1 6 wasp (Addgene inc)
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prrl ws1 6 wasp
Prrl Ws1 6 Wasp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prrl+ws1+6+wasp/pRRL+WS1%2E6+WASp+(Plasmid+%2336250)/pmc12575061__10-1055-a-2508-0983-s24070322-19-61-62
Average 93 stars, based on 1 article reviews
Prrl Ws1 6 Wasp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prrl+ws1+6+wasp/pRRL+WS1%2E6+WASp+(Plasmid+%2336250)/pmc12575061__10-1055-a-2508-0983-s24070322-19-61-62
Average 93 stars, based on 1 article reviews
prrl ws1 6 wasp - by Bioz Stars,
2026-10
93/100 stars
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Plasmid Preparation:Article Title: Gene correction of Wiskott-Aldrich-syndrome iPS cells rescues proplatelet defects and improves platelet size. Article Snippet: AAVS1 gene targeting design For targeting WASP expression cassette at the AAVS1 site, hAAVS1L TALEN (Addgene plasmid # 35431) and hAAVS1R TALEN (Addgene plasmid # 35432), gifts from Feng Zhang, were used to introduce DNA DSB at the target region. .. For donor vector construction, the MNDWASP and the WAS1.6-WASP were amplified from pRRL-MND-WASp (Addgene plasmid # 36248) and Article Title: Gene correction of Wiskott-Aldrich-syndrome iPS cells rescues proplatelet defects and improves platelet size. Article Snippet: AAVS1 gene targeting design For targeting WASP expression cassette at the AAVS1 site, hAAVS1L TALEN (Addgene plasmid # 35431) and hAAVS1R TALEN (Addgene plasmid # 35432), gifts from Feng Zhang, were used to introduce DNA DSB at the target region21. .. For donor vector construction, the MNDWASP and the WAS1.6-WASP were amplified from pRRL-MND-WASp (Addgene plasmid # 36248) and Article Title: Gene Correction of Wiskott–Aldrich syndrome iPS Cells Rescues Proplatelet Defects and Improves Platelet Size Article Snippet: For WASQ19X gene correction using the CRISPR/Cas9 system, 20-bp sgRNA targeting 3-nt upstream of themutation sitewas designed and cloned into the pSpCas9 (BB)-2A-GFP (px458; a gift fromFeng Zhang, Addgene plasmid # 48138).40 A 90-bp ssODN with a silence mutation at positions 15 and 18 of the gRNA-binding site was used as a template for homologous recombination (HR). .. AAVS1 Gene Targeting Design For targeting theWiskott–Aldrich syndrome protein (WASP) expression cassette at the AAVS1 site, hAAVS1L TALEN (Addgene plasmid # 35431) and hAAVS1R TALEN (Addgene plasmid # 35432), gifts from Feng Zhang, were used to introduce DNA DSB at the target region.21 For donor vector construction, the MND-WASP and the WAS1.6-WASP were amplified from pRRL-MND-WASp (Addgene plasmid # 36248) and Amplification:Article Title: Gene correction of Wiskott-Aldrich-syndrome iPS cells rescues proplatelet defects and improves platelet size. Article Snippet: AAVS1 gene targeting design For targeting WASP expression cassette at the AAVS1 site, hAAVS1L TALEN (Addgene plasmid # 35431) and hAAVS1R TALEN (Addgene plasmid # 35432), gifts from Feng Zhang, were used to introduce DNA DSB at the target region. .. For donor vector construction, the MNDWASP and the WAS1.6-WASP were amplified from pRRL-MND-WASp (Addgene plasmid # 36248) and Article Title: Gene correction of Wiskott-Aldrich-syndrome iPS cells rescues proplatelet defects and improves platelet size. Article Snippet: AAVS1 gene targeting design For targeting WASP expression cassette at the AAVS1 site, hAAVS1L TALEN (Addgene plasmid # 35431) and hAAVS1R TALEN (Addgene plasmid # 35432), gifts from Feng Zhang, were used to introduce DNA DSB at the target region21. .. For donor vector construction, the MNDWASP and the WAS1.6-WASP were amplified from pRRL-MND-WASp (Addgene plasmid # 36248) and Article Title: Gene Correction of Wiskott–Aldrich syndrome iPS Cells Rescues Proplatelet Defects and Improves Platelet Size Article Snippet: For WASQ19X gene correction using the CRISPR/Cas9 system, 20-bp sgRNA targeting 3-nt upstream of themutation sitewas designed and cloned into the pSpCas9 (BB)-2A-GFP (px458; a gift fromFeng Zhang, Addgene plasmid # 48138).40 A 90-bp ssODN with a silence mutation at positions 15 and 18 of the gRNA-binding site was used as a template for homologous recombination (HR). .. AAVS1 Gene Targeting Design For targeting theWiskott–Aldrich syndrome protein (WASP) expression cassette at the AAVS1 site, hAAVS1L TALEN (Addgene plasmid # 35431) and hAAVS1R TALEN (Addgene plasmid # 35432), gifts from Feng Zhang, were used to introduce DNA DSB at the target region.21 For donor vector construction, the MND-WASP and the WAS1.6-WASP were amplified from pRRL-MND-WASp (Addgene plasmid # 36248) and Clone Assay:Article Title: Gene correction of Wiskott-Aldrich-syndrome iPS cells rescues proplatelet defects and improves platelet size. Article Snippet: AAVS1 gene targeting design For targeting WASP expression cassette at the AAVS1 site, hAAVS1L TALEN (Addgene plasmid # 35431) and hAAVS1R TALEN (Addgene plasmid # 35432), gifts from Feng Zhang, were used to introduce DNA DSB at the target region. .. For donor vector construction, the MNDWASP and the WAS1.6-WASP were amplified from pRRL-MND-WASp (Addgene plasmid # 36248) and Article Title: Gene correction of Wiskott-Aldrich-syndrome iPS cells rescues proplatelet defects and improves platelet size. Article Snippet: AAVS1 gene targeting design For targeting WASP expression cassette at the AAVS1 site, hAAVS1L TALEN (Addgene plasmid # 35431) and hAAVS1R TALEN (Addgene plasmid # 35432), gifts from Feng Zhang, were used to introduce DNA DSB at the target region21. .. For donor vector construction, the MNDWASP and the WAS1.6-WASP were amplified from pRRL-MND-WASp (Addgene plasmid # 36248) and Article Title: Gene Correction of Wiskott–Aldrich syndrome iPS Cells Rescues Proplatelet Defects and Improves Platelet Size Article Snippet: For WASQ19X gene correction using the CRISPR/Cas9 system, 20-bp sgRNA targeting 3-nt upstream of themutation sitewas designed and cloned into the pSpCas9 (BB)-2A-GFP (px458; a gift fromFeng Zhang, Addgene plasmid # 48138).40 A 90-bp ssODN with a silence mutation at positions 15 and 18 of the gRNA-binding site was used as a template for homologous recombination (HR). .. AAVS1 Gene Targeting Design For targeting theWiskott–Aldrich syndrome protein (WASP) expression cassette at the AAVS1 site, hAAVS1L TALEN (Addgene plasmid # 35431) and hAAVS1R TALEN (Addgene plasmid # 35432), gifts from Feng Zhang, were used to introduce DNA DSB at the target region.21 For donor vector construction, the MND-WASP and the WAS1.6-WASP were amplified from pRRL-MND-WASp (Addgene plasmid # 36248) and Bioprocessing:Article Title: Gene correction of Wiskott-Aldrich-syndrome iPS cells rescues proplatelet defects and improves platelet size. Article Snippet: AAVS1 gene targeting design For targeting WASP expression cassette at the AAVS1 site, hAAVS1L TALEN (Addgene plasmid # 35431) and hAAVS1R TALEN (Addgene plasmid # 35432), gifts from Feng Zhang, were used to introduce DNA DSB at the target region. .. For donor vector construction, the MNDWASP and the WAS1.6-WASP were amplified from pRRL-MND-WASp (Addgene plasmid # 36248) and Article Title: Gene correction of Wiskott-Aldrich-syndrome iPS cells rescues proplatelet defects and improves platelet size. Article Snippet: AAVS1 gene targeting design For targeting WASP expression cassette at the AAVS1 site, hAAVS1L TALEN (Addgene plasmid # 35431) and hAAVS1R TALEN (Addgene plasmid # 35432), gifts from Feng Zhang, were used to introduce DNA DSB at the target region21. .. For donor vector construction, the MNDWASP and the WAS1.6-WASP were amplified from pRRL-MND-WASp (Addgene plasmid # 36248) and Article Title: Gene Correction of Wiskott–Aldrich syndrome iPS Cells Rescues Proplatelet Defects and Improves Platelet Size Article Snippet: For WASQ19X gene correction using the CRISPR/Cas9 system, 20-bp sgRNA targeting 3-nt upstream of themutation sitewas designed and cloned into the pSpCas9 (BB)-2A-GFP (px458; a gift fromFeng Zhang, Addgene plasmid # 48138).40 A 90-bp ssODN with a silence mutation at positions 15 and 18 of the gRNA-binding site was used as a template for homologous recombination (HR). .. AAVS1 Gene Targeting Design For targeting theWiskott–Aldrich syndrome protein (WASP) expression cassette at the AAVS1 site, hAAVS1L TALEN (Addgene plasmid # 35431) and hAAVS1R TALEN (Addgene plasmid # 35432), gifts from Feng Zhang, were used to introduce DNA DSB at the target region.21 For donor vector construction, the MND-WASP and the WAS1.6-WASP were amplified from pRRL-MND-WASp (Addgene plasmid # 36248) and Expressing:Article Title: Gene Correction of Wiskott–Aldrich syndrome iPS Cells Rescues Proplatelet Defects and Improves Platelet Size Article Snippet: For WASQ19X gene correction using the CRISPR/Cas9 system, 20-bp sgRNA targeting 3-nt upstream of themutation sitewas designed and cloned into the pSpCas9 (BB)-2A-GFP (px458; a gift fromFeng Zhang, Addgene plasmid # 48138).40 A 90-bp ssODN with a silence mutation at positions 15 and 18 of the gRNA-binding site was used as a template for homologous recombination (HR). .. AAVS1 Gene Targeting Design For targeting theWiskott–Aldrich syndrome protein (WASP) expression cassette at the AAVS1 site, hAAVS1L TALEN (Addgene plasmid # 35431) and hAAVS1R TALEN (Addgene plasmid # 35432), gifts from Feng Zhang, were used to introduce DNA DSB at the target region.21 For donor vector construction, the MND-WASP and the WAS1.6-WASP were amplified from pRRL-MND-WASp (Addgene plasmid # 36248) and Introduce:Article Title: Gene Correction of Wiskott–Aldrich syndrome iPS Cells Rescues Proplatelet Defects and Improves Platelet Size Article Snippet: For WASQ19X gene correction using the CRISPR/Cas9 system, 20-bp sgRNA targeting 3-nt upstream of themutation sitewas designed and cloned into the pSpCas9 (BB)-2A-GFP (px458; a gift fromFeng Zhang, Addgene plasmid # 48138).40 A 90-bp ssODN with a silence mutation at positions 15 and 18 of the gRNA-binding site was used as a template for homologous recombination (HR). .. AAVS1 Gene Targeting Design For targeting theWiskott–Aldrich syndrome protein (WASP) expression cassette at the AAVS1 site, hAAVS1L TALEN (Addgene plasmid # 35431) and hAAVS1R TALEN (Addgene plasmid # 35432), gifts from Feng Zhang, were used to introduce DNA DSB at the target region.21 For donor vector construction, the MND-WASP and the WAS1.6-WASP were amplified from pRRL-MND-WASp (Addgene plasmid # 36248) and |